Journal of Asia Pacific Aesthetic Sciences

Abstract: Platelets are important for hemostasis and wound healing. Several growth factors have been discovered in alpha granules [Tsay, el al., 2005]. Platelet-rich plasma (PRP) is obtained by centrifuging autologous blood and isolating the platelets. As a result, PRP devices are generally divided into two concentration systems: lower (2.5–3 times baseline concentration) and higher (5–9 times baseline concentration) [Dhurat,&Sukesh, 2014]. Conventionally, PRP is prepared either through commercial kits or a specialized ‘table top cold centrifuge’ device in the clinic set-up. 30 ml of venous blood draw will yield approximately 3-5 cc of PRP depending on the baseline platelet count of an individual, the device used, and the technique employed such as using citrate dextrose A to prevent platelet activation prior to its use. However, in clinical practice, the platelet levels are not measured in the aforementioned conventional method. Lyophilized platelet-rich plasma is a technology where isolated and purified PRP are stored for a prolonged period. Generally, they are sent to the central laboratory with proper environment control for centrifugation and further processing. The lyophilized PRP is then checked for sterility and sent back to the medical facility for administration. Before each treatment session, the lyophilized PRP can be simply reconstituted with normal saline or other activator such as collagen [Murdiastuti, et al., 2019]. PRP has been employed in a variety of medical applications, including tendon repair, reconstructive medicine, wound healing, hair loss, and aesthetic medicine. Furthermore, application of PRP in medical aesthetic also indicate improvement for skin texture, wrinkles, mild collagen loss, skin tightening and toning, acne scars, and face volume in previous studies [Frautschi, et al., 2017; Peng, 2019; Samadi. Et al., 2019].